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borosilicate thick walled glass capillary tubing  (Sutter Instrument Company)


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    Sutter Instrument Company borosilicate thick walled glass capillary tubing
    Borosilicate Thick Walled Glass Capillary Tubing, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 98/100, based on 3829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thick-walled+borosilicate+glass+capillary+tubes/Borosilicate+Glass/pm41002424-45-4-9
    Average 98 stars, based on 3829 article reviews
    borosilicate thick walled glass capillary tubing - by Bioz Stars, 2026-10
    98/100 stars

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    Patch Clamp:

    Article Title: Protons and Psalmotoxin-1 reveal nonproton ligand stimulatory sites in chicken acid-sensing ion channel
    Article Snippet: .. Whole cell and outside-out patch-clamp recordings were obtained using thick-walled borosilicate glass capillary tubes (Sutter Instrument Company), which were pulled to a resistance of 3–6 MΩ (Flaming/Brown, P-87/PC, Sutter Instrument Co.) and subsequently fire-polished. ..

    Microinjection:

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats
    Article Snippet: .. Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ). .. Tips were filled with AMPA (Sigma), mixed daily from stock solution, connected to a picospritzer (Picospritzer II, General Valve), and mounted on a micromanipulator for injection; a stereomicroscope mounted above the recording chamber was used to visualize tip placement.



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    XIIn activity after <t>AMPA</t> microinjection into the XII motor nucleus. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the XIIMN. A, B, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 1 μM AMPA into the XIIMN (arrow indicates injection start time). Boxed regions in A and B are expanded in C and D, respectively. E, Tonic activity amplitude, calculated as percentage change from the preinjection baseline for control and DNE preparations (control, 483.36 ± 119.84%; DNE, 658.11 ± 180.43%; p = 0.484). The amplitude of tonic activity was measured in the periods between phasic XIIn bursts. F, Duration of increased tonic activity after AMPA injection in control and DNE preparations (control, 14.03 ± 1.68 s; DNE, 40.18 ± 7.43 s; p = 0.012). Error bars represent SEM.
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    XIIn activity after AMPA microinjection into the XII motor nucleus. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the XIIMN. A, B, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 1 μM AMPA into the XIIMN (arrow indicates injection start time). Boxed regions in A and B are expanded in C and D, respectively. E, Tonic activity amplitude, calculated as percentage change from the preinjection baseline for control and DNE preparations (control, 483.36 ± 119.84%; DNE, 658.11 ± 180.43%; p = 0.484). The amplitude of tonic activity was measured in the periods between phasic XIIn bursts. F, Duration of increased tonic activity after AMPA injection in control and DNE preparations (control, 14.03 ± 1.68 s; DNE, 40.18 ± 7.43 s; p = 0.012). Error bars represent SEM.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: XIIn activity after AMPA microinjection into the XII motor nucleus. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the XIIMN. A, B, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 1 μM AMPA into the XIIMN (arrow indicates injection start time). Boxed regions in A and B are expanded in C and D, respectively. E, Tonic activity amplitude, calculated as percentage change from the preinjection baseline for control and DNE preparations (control, 483.36 ± 119.84%; DNE, 658.11 ± 180.43%; p = 0.484). The amplitude of tonic activity was measured in the periods between phasic XIIn bursts. F, Duration of increased tonic activity after AMPA injection in control and DNE preparations (control, 14.03 ± 1.68 s; DNE, 40.18 ± 7.43 s; p = 0.012). Error bars represent SEM.

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Activity Assay, Microinjection, Control, Injection

    Burst characteristics in control and DNE slices after AMPA microinjection into the XIIMN. Comparisons of burst characteristics between control (n = 8) and DNE (n = 11) preparations for the first 25 postinjection bursts (1 μM AMPA injected for 5 s). Burst characteristics reported as the percentage change from baseline bursts averaged over a 2 min period (100%, hyphenated line). Characteristics measured included the following. A, Peak burst amplitude. B, Average burst amplitude. C, Burst duration. D, Burst area. ***p < 0.0001 (control vs DNE). **p < 0.01 (control vs DNE).

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: Burst characteristics in control and DNE slices after AMPA microinjection into the XIIMN. Comparisons of burst characteristics between control (n = 8) and DNE (n = 11) preparations for the first 25 postinjection bursts (1 μM AMPA injected for 5 s). Burst characteristics reported as the percentage change from baseline bursts averaged over a 2 min period (100%, hyphenated line). Characteristics measured included the following. A, Peak burst amplitude. B, Average burst amplitude. C, Burst duration. D, Burst area. ***p < 0.0001 (control vs DNE). **p < 0.01 (control vs DNE).

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Control, Microinjection, Injection

    AMPA microinjection. A, Nissl-stained section with critical regions of interest highlighted on the left, and a diagram of pipette tip placement and the recording setup on the right. 4V, Fourth ventricle; Inf. Olive, Inferior Olive. B, Representative electroneurogram (ENG) obtained from the XIIn; boxed region in the top panel is expanded below to show the raw and integrated representation of one respiratory-related XIIn burst. The variables measured for each of these bursts are denoted on the integrated ENG trace. Average amplitude (measurement not shown) was obtained by dividing the burst area by the burst duration. C, Example of a microinjection site (arrow) in the XIIMN in a Nissl-stained section (original magnification 20×).

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: AMPA microinjection. A, Nissl-stained section with critical regions of interest highlighted on the left, and a diagram of pipette tip placement and the recording setup on the right. 4V, Fourth ventricle; Inf. Olive, Inferior Olive. B, Representative electroneurogram (ENG) obtained from the XIIn; boxed region in the top panel is expanded below to show the raw and integrated representation of one respiratory-related XIIn burst. The variables measured for each of these bursts are denoted on the integrated ENG trace. Average amplitude (measurement not shown) was obtained by dividing the burst area by the burst duration. C, Example of a microinjection site (arrow) in the XIIMN in a Nissl-stained section (original magnification 20×).

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Microinjection, Staining, Transferring

    Burst characteristics in control and DNE after AMPA microinjection into the pre-BötC region. Comparisons of burst characteristics between control (n = 9) and DNE (n = 9) for the first 25 postinjection bursts (10 μM AMPA injected for 5 s). Burst characteristics reported as percentage change from preinjection baseline values that were averaged over a 2 min period (100%, hyphenated line). Characteristics measured included the following. A, Peak burst amplitude. B, Average burst amplitude. C, Burst duration. D, Burst area. ***p < 0.0001 (control vs DNE).

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: Burst characteristics in control and DNE after AMPA microinjection into the pre-BötC region. Comparisons of burst characteristics between control (n = 9) and DNE (n = 9) for the first 25 postinjection bursts (10 μM AMPA injected for 5 s). Burst characteristics reported as percentage change from preinjection baseline values that were averaged over a 2 min period (100%, hyphenated line). Characteristics measured included the following. A, Peak burst amplitude. B, Average burst amplitude. C, Burst duration. D, Burst area. ***p < 0.0001 (control vs DNE).

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Control, Microinjection, Injection

    Changes in burst frequency after AMPA microinjection into XIIMN and pre-BötC region. Frequency of XIIn bursts for the first 25 postinjection bursts is reported as percentage change from the average burst frequency in the 2 min preinjection period (100%, hyphenated line). A, Comparison of spontaneous bursting frequency between control (n = 8) and DNE (n = 11) after XIIMN microinjections (1 μM AMPA, 5 s). Inset, The same graph on an expanded time scale for the first 10 bursts for a more detailed comparison. B, Comparisons of spontaneous bursting frequency between control (n = 9) and DNE (n = 9) after pre-BötC microinjections (10 μM AMPA, 5 s). ***p < 0.0001 (control vs DNE).

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: Changes in burst frequency after AMPA microinjection into XIIMN and pre-BötC region. Frequency of XIIn bursts for the first 25 postinjection bursts is reported as percentage change from the average burst frequency in the 2 min preinjection period (100%, hyphenated line). A, Comparison of spontaneous bursting frequency between control (n = 8) and DNE (n = 11) after XIIMN microinjections (1 μM AMPA, 5 s). Inset, The same graph on an expanded time scale for the first 10 bursts for a more detailed comparison. B, Comparisons of spontaneous bursting frequency between control (n = 9) and DNE (n = 9) after pre-BötC microinjections (10 μM AMPA, 5 s). ***p < 0.0001 (control vs DNE).

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Microinjection, Comparison, Control

    XIIn activity after AMPA microinjection into the pre-BötC region. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the pre-BötC region. A, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 10 μM AMPA into the pre-BötC for 5 s (arrow indicates injection start time). Underlined regions in A and B are expanded in C and D, respectively. Note that injection of AMPA increased nerve burst frequency in both control and DNE preparations and that the increase was greater in the DNE slices.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: XIIn activity after AMPA microinjection into the pre-BötC region. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the pre-BötC region. A, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 10 μM AMPA into the pre-BötC for 5 s (arrow indicates injection start time). Underlined regions in A and B are expanded in C and D, respectively. Note that injection of AMPA increased nerve burst frequency in both control and DNE preparations and that the increase was greater in the DNE slices.

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Activity Assay, Microinjection, Control, Injection

    Peak inward current and recovery time constant of XII motoneurons after AMPA microinjection. Voltage-clamp data from hypoglossal motoneurons in rhythmic brainstem slices from control and DNE preparations. A, Representative traces of inward currents obtained from control and DNE hypoglossal motoneurons in response to microinjection of 1 μM AMPA at a distance of 50 μm from the recorded cell. Bottom, Response of a control motoneuron to the AMPA puff, but in the presence of 10 μM CNQX, an AMPA receptor antagonist. CNQX blocked the majority of the response, indicating that the currents were AMPA-mediated. B, C, Average peak inward currents and recovery time constant in neurons from both treatment groups (control, n = 10 cells; DNE, n = 14 cells). No significant differences were found between treatment groups for either peak inward current or the recovery time constant.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: Peak inward current and recovery time constant of XII motoneurons after AMPA microinjection. Voltage-clamp data from hypoglossal motoneurons in rhythmic brainstem slices from control and DNE preparations. A, Representative traces of inward currents obtained from control and DNE hypoglossal motoneurons in response to microinjection of 1 μM AMPA at a distance of 50 μm from the recorded cell. Bottom, Response of a control motoneuron to the AMPA puff, but in the presence of 10 μM CNQX, an AMPA receptor antagonist. CNQX blocked the majority of the response, indicating that the currents were AMPA-mediated. B, C, Average peak inward currents and recovery time constant in neurons from both treatment groups (control, n = 10 cells; DNE, n = 14 cells). No significant differences were found between treatment groups for either peak inward current or the recovery time constant.

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Microinjection, Control

    Spike frequency in XII motoneurons after AMPA microinjection. Current-clamp recordings from control and DNE XII motoneurons from rhythmic brainstem slices in response to AMPA injection into the XIIMN (control, n = 12 cells; DNE, n = 7 cells). A, Representative current-clamp recordings from a control and DNE XII motoneuron (spike frequency, control, 7.9 Hz; and DNE, 18.4 Hz). B, Average spike frequency data obtained from all cells in response to AMPA puff. ***p < 0.0001 (control vs DNE). C, Example of a current-clamp recording obtained from a rhythmically active XII motoneuron that demonstrated a large increase in Vm in response to AMPA injection, leading to depolarization block.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats

    doi: 10.1523/JNEUROSCI.3711-12.2013

    Figure Lengend Snippet: Spike frequency in XII motoneurons after AMPA microinjection. Current-clamp recordings from control and DNE XII motoneurons from rhythmic brainstem slices in response to AMPA injection into the XIIMN (control, n = 12 cells; DNE, n = 7 cells). A, Representative current-clamp recordings from a control and DNE XII motoneuron (spike frequency, control, 7.9 Hz; and DNE, 18.4 Hz). B, Average spike frequency data obtained from all cells in response to AMPA puff. ***p < 0.0001 (control vs DNE). C, Example of a current-clamp recording obtained from a rhythmically active XII motoneuron that demonstrated a large increase in Vm in response to AMPA injection, leading to depolarization block.

    Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).

    Techniques: Microinjection, Control, Injection, Blocking Assay